What methods can be used to identify chemolithotrophs in 16S data?

Question

[will.wcb]

I have a large 16S data set, and I am looking to identify which samples have the largest proportion of chemolithotrophs. I was wondering if anyone has any ideas on how we might be able to identify those taxa which are most likely to be chemolithotrophs.

Thank you

[h.mon]

I can only think of extensive literature review.

Answer 1

[Mensur Dlakic]

One way to do it is to add 16S rRNA sequences of know chemolithotrophs and non-chemolithotrophs to your data, and build a phylogenetic tree. The sequences from your data that group together with know chemolithotrophs are more likely to be in the same category.