Graphics For Sequencing, Mapping

Question

[Daniel Standage]

I'm preparing a presentation on an algorithm for processing RNA-seq data. The audience will have a varied background, but I don't want to assume more than basic experience with biology and DNA sequencing. I'm having a hard time finding graphics to supplement this presentation. Any pointers? Here is what I'm looking for.

• A graphic describing how ESTs are sequenced in an RNA-seq experiment (I understand this is very specific, so a good graphic describing the sequencing process in general might work here)
• A graphic showing ESTs mapped back to a reference genome.

Thanks.

Update: Thanks for the responses, although we didn't end up including any of the suggested pictures. This was a really hard presentation to find pictures for. I make it a point to reduce text and increase visuals in my presentations, so this one didn't turn out as well as I had hoped. Anyway, you can find it here. Let me know your thoughts.

[Alastair Kerr]

Could you please post the presentation back to this forum when it is done? It would be nice to have more resources like this available.

[Daniel Standage]

Sure. I'm not going to promise much, but I'll share what we come up with!

Answer 1

[Pierre Lindenbaum]

There are some graphics in the article "RNA seq" in wikipedia: http://en.wikipedia.org/wiki/RNA_seq

Answer 2

[Mary]

Check out this paper, and see if Figure 2C suits one your needs. I think it's effective, but I sorta am not your target for this probably.

Dynamic transcriptomes during neural differentiation of human embryonic stem cells revealed by short, long, and paired-end sequencing

EDIT: here's another one I found helpful getting started in this area. See Figure 1: Mapping and quantifying mammalian transcriptomes by RNA-Seq