Upstream regulators of a gene/protein

Question

[chipolino]

Hi,

Suppose I have a protein of interest. And my goal is to identify its upstream regulators (activators, inhibitors, etc.). Is there any software that allows to do it? Or is it feasible to only look at transcription factors that regulate the expression of the corresponding gene?

Thank you

Answer 1

[piyushjo]

One way to do it is to use STRINGdb. They have various type of interaction information. The annotation will also tell you what the presumed interaction is and the level of evidence.

The second is to use Ingeniuty Pathway Analysis. However, this is a paid service. You can get similar information, but less detailed, from NCBI search for the "Gene" or use genecards.org. Those two will have more information based on GO terms and pathways known to be affected/regulated by a factor.

Hope it helps!