abundance_estimates_to_matrix.pl error while processing kallisto output

Question

[Shahzad]

Dear All please help me to solve this error.

perl abundance_estimates_to_matrix.pl --est_method kallisto --name_sample_by_basedir 1rc/1rc.tsv 2rc/2rc.tsv 3rc/3rc.tsv 1rt/1rt.tsv 2rt/2rt.tsv 3rT/3rt.tsv 1sc/1sc.tsv 2sc/2sc.tsv 3sc/3sc.tsv 1st/1st.tsv 2ST/2st.tsv 3st/3st.tsv --out_prefix guided-matrix --gene_trans_map none
-reading file: 1rc/1rc.tsv
-reading file: 2rc/2rc.tsv
-reading file: 3rc/3rc.tsv
-reading file: 1rt/1rt.tsv
-reading file: 2rt/2rt.tsv
-reading file: 3rT/3rt.tsv
-reading file: 1sc/1sc.tsv
-reading file: 2sc/2sc.tsv
-reading file: 3sc/3sc.tsv
-reading file: 1st/1st.tsv
-reading file: 2ST/2st.tsv
-reading file: 3st/3st.tsv


* Outputting combined matrix.

/home/ubuntu/Desktop/deg/support_scripts/run_TMM_scale_matrix.pl --matrix guided-matrix.isoform.TPM.not_cross_norm > guided-matrix.isoform.TMM.EXPR.matrixsh: 1: /home/ubuntu/Desktop/deg/support_scripts/run_TMM_scale_matrix.pl: not found
Error, CMD: /home/ubuntu/Desktop/deg/support_scripts/run_TMM_scale_matrix.pl --matrix guided-matrix.isoform.TPM.not_cross_norm > guided-matrix.isoform.TMM.EXPR.matrix died with ret 32512 at bundance_estimates_to_matrix.pl line 372.

[ATpoint]

/home/ubuntu/Desktop/deg/support_scripts/run_TMM_scale_matrix.pl: not found

This script is not present at a location where this tool expects it.

[Shahzad]

is there any link to this file "run_TMM_scale_matrix.pl" . i am unable to find it in trinity pkg files

[prince26121991]

Here is the default path of run_TMM_scale_matrix.pl

trinityrnaseq/util/support_scripts/run_TMM_scale_matrix.pl

trinityrnaseq