Entering edit mode
5.3 years ago
biosol
▴
170
Hi everyone,
I'm trying to analyse mitochondrial data from human samples, which I have stored in a vcf
file. I've tried running Haplogrep2
(both cmd and online) but my results do not make sense. Reading other posts, I've realised that I should first align my data to the rCRS
sequence, so I was wondering which tool to use... can please anyone suggest one? Or does Haplogrep2 directly align vcfs to the reference sequence?
I have another doubt as well: my vcf
has 0/0
and 1/1
in every position, but... isn't mitochondrial DNA a single molecule?? Shouldn't I only have one allele??
Thanks in advance!
Haplogrep only supports rCRS/RSRS in the chromosome field in input files(VCF). The tool that has been used to call the variants uses ploidy 2 by default thats why you get 0/0 and 1/1.