assembler for viral genome sequencing data
0
0
Entering edit mode
5.2 years ago

I have raw reads of virus data. and I want to assemble the viral genome. any tool / pipeline suggestion and also help me regarding viral annotation as well

assembly virus genome • 2.7k views
ADD COMMENT
0
Entering edit mode

Is it genome sequencing or metagenomic dataset? In my experience, SPAdes works well for viral genome assemblies.

ADD REPLY
0
Entering edit mode

this is genome sequencing. I just tried SPAdes but not a good assembly results

ADD REPLY
0
Entering edit mode

What do you mean by a not good assembly? What other pre-processing steps have you performed, e.g. adapter trimming, removing host sequences. It'd be advisable to provide as much information as possible including the sequencing platform, the type of sequencing and a bit about the samples itself. These details would help understand this specific problem better.

For more info on how to ask a good question please read: [ Please read before posting a question ] -- How To Ask A Good Question

ADD REPLY
0
Entering edit mode

If you know which virus it is you could try to find a reference and use BWA

ADD REPLY
0
Entering edit mode

tadpole.sh from BBMap suite works well for small/viral genomes. A guide is available here.

ADD REPLY

Login before adding your answer.

Traffic: 1535 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6