remote blast requires installation of taxdb database
0
3
Entering edit mode
7.1 years ago
hfan22 ▴ 40

I was trying to do some remote blast. Here is my command:

blastn -query myquery.fasta -db nt -out myblast.out -remote -num_alignments 10 -outfmt "6 qacc saccver pident sscinames length mismatch gapopen evalue bitscore"

I wanted to get the scientific name instead of taxid so I requested sscinames in my output format. I am almost certain that this worked for me a few months ago (according to my work log). Today it stopped working, returning N/A for the sscinames column with warning,

Warning: [blastn] Taxonomy name lookup from taxid requires installation of taxdb database with ftp://ftp.ncbi.nlm.nih.gov/blast/db/taxdb.tar.gz

for each query.

I would assume installing taxdb on my local computer wouldn't help.

I am using blast 2.6.0+

Any help is appreciated.

blast • 10k views
ADD COMMENT
0
Entering edit mode

Have you emailed NCBI blast support if you are sure this has worked in the past? Perhaps there may just be some temporary glitch. They are generally responsive.

ADD REPLY
0
Entering edit mode

I have the same problem. Did you find a solution?

ADD REPLY
0
Entering edit mode

I also have this problem Is there any update?

ADD REPLY
0
Entering edit mode

Did you download ftp://ftp.ncbi.nlm.nih.gov/blast/db/taxdb.tar.gz, uncompress and try putting it in the same folder as other blast indexes?

ADD REPLY
0
Entering edit mode

yes I did and also updated to blast 2.9 (I had 2.7). From NCBI they answered that the taxdb is required by 'sscinames' so I skipped that. I solved by grepping the taxonomy ID from the taxdb file. Less elegant but it works...

ADD REPLY
0
Entering edit mode

Hi,

I had the same issue when I was using the remote option, I found the solution from here: getting sscinames in viral blastx query and it worked for me...

Hope this will help you.

Thanks

ADD REPLY
0
Entering edit mode

Did you have any luck with this?

Thanks

ADD REPLY

Login before adding your answer.

Traffic: 1865 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6