hello everyone; What is the difference between pre-processing and normalization in one color and two color Agilent Microarray data? please suggest best package R For both
Two-channel (color) microarrays are usually normalized with LOESS, but one-channel arrays usually only with a between-array method (like quantile). The best tool to use is limma, you can read in the manual how to normalize and analyze these different array types, including case studies with scripting codes.
Limma. Please read its manual carefully. It extensively covers analysis of these types of arrays.
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version 2.3.6
As per Benn:
in the limma, it has two different part of two color, one is direct two-color, do you know the meanning
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