Entering edit mode
5.1 years ago
MutationalMeltdown
▴
200
I've ran kallisto bus
on multiple read pairs (R1 and R2) fastq files (scRNA-seq 10x data) and I'm trying to understand the QC metrics in run_info.json
:
"n_targets": 0,
"n_bootstraps": 0,
"n_processed": 286634021,
"n_pseudoaligned": 8387513897523690476,
"n_unique": 50123568,
"p_pseudoaligned": 2926210178492.3,
"p_unique": 17.5,
"kallisto_version": "0.46.0",
"index_version": 0,
I read here what the metrics mean: http://pachterlab.github.io/kallisto//releases/2018/01/29/v0.44.0
However, for my data, p_pseudoaligned
is >>100% and n_pseudoaligned
>> n_processed
. What I expected was that p_pseudoaligned
would be somewhat analagous to % read alignment/mapping with a 'normal' aligner. What am I missing? Thank you
EDIT: posted to forum
Please re-post at the Kallisto Google Group page, and kindly link back here (and vice-versa) - this will help the community: https://groups.google.com/forum/?nomobile=true#!forum/kallisto-sleuth-users
I posted to the "kallisto-and-applications" forum