downloading promoter sequences using UCSC Table Browser
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5.1 years ago
ravi.eshwari ▴ 10

HI, I need to download 2000 upstream promoter region sequences of few genes but when i specify the gene along with its coordinates and chromosome the output sequence file i get contains all other coordinates of those genes except the one i mentioned in the input how do i download exact coordinate sequence file? please let me know

thankyou!!

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You can simply obtain the coordinates of the entire gene and then use bedtools slop to extend the start of the gene by a desired number of basepairs in upstream direction to get an approximation of the promoter sequence. Be sure to use it in a strand-specific fashion to respect the strand the gene is on.

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