Dear all,
I am doing de novo assembly (aspergillus flavus) in clc genomics workbench when i export assembled file in fasta format i am getting error.
Export AGP
Export error: The scaffold region did not cover a region with N's (Annotation name: Scaffold Type: Scaffold: Region: 12036..12037 in CI21_(paired)_contig_2
1/ this may have been a bug at the time if you were trying to directly export a de novo assembly to FASTA. The work around was to first call consensus contigs from the assembly graph, and then export that.
2/ in the latest version (GxWB12 as of this writing) you can dump FASTA directly from assembly results without issue.
Since you are using commercial software the company should also offer you support on these issues.
Okay, but i am using trail version