Entering edit mode
5.1 years ago
mgpastos
▴
10
Hi everyone.
I'm working with sdRADseq data and I am trying to do the reference assembly pipeline.
At step 3, I got the next error
Step 3: Clustering/Mapping reads within samples
[####################] 100% 0:28:32 | indexing reference
[####################] 100% 0:00:01 | concatenating
[####################] 100% 0:00:40 | dereplicating
[####################] 100% 0:11:09 | mapping reads
Encountered an Error.
Message: IPyradError: bwa error: None
Use debug flag (-d) for full code traceback.
I'm working with ipyrad [v.0.7.30] on a HPC cluster. I don't know why I can do to solve that.
using de -d flag I get this:
python2.7/site-packages/bin/bedtools-linux-x86_64', 'bamtobed', '-i', '/panfs/roc/groups/14/mcgaughs/mgarduos/ipyrad/radseq_refmapping/TRIM_T7548_S8001-mapped-sorted.bam'] ['/home/mcgaughs/mgarduos/software/ls/lib/python2.7/site-packages/bin/bedtools-linux-x86_64', 'merge', '-d', '-10', '-i', '-']
2019-10-14 14:45:07,857 pid=24965 [assembly.py] ERROR IPyradError(None)
2019-10-14 14:45:08,908 pid=24965 [assembly.py] INFO interrupted engine 2 w/ SIGINT to 25036
2019-10-14 14:45:08,937 pid=24965 [assembly.py] INFO interrupted engine 12 w/ SIGINT to 25152
2019-10-14 14:45:08,958 pid=24965 [assembly.py] INFO interrupted engine 18 w/ SIGINT to 25225
2019-10-14 14:45:09,976 pid=24965 [assembly.py] INFO shutting down engines
2019-10-14 14:45:10,029 pid=24965 [assembly.py] INFO finished shutdown
2019-10-14 14:45:10,033 pid=24965 [__init__.py] INFO debugging turned off
I hope that someone could help me with mi issue.