Entering edit mode
5.1 years ago
prasundutta87
▴
670
It is known that BWA-MEM soft-clips bases to achieve a higher alignment rate. If a variant caller is used after that, say, GATK Haplotyecaller, is it necessary to keep the parameter --dont-use-soft-clipped-bases to TRUE? Or should we keep it in default (false) as it may affect the realignment step during Haplotycaller run?
Please stick to the GATK best practices. If they don't recommend to use that parameter there is no need to overthink.