Deferentially Expressed Genes using Genes count data obtained from RNASeq

Question

[naseerkhan861]

I have obtained Genes Count data after the analysis of RNASeq dataset , Now I want to analyze my dataset and extract DEGs(Differentially Expressed Genes) as my end goal is to perform clustering using WGCA once I have the DEGs from my dataset, Can somebody guide me how to proceed or guide me any link that I can follow accordingly. Also is it a good way to proceed like this I mean first finding DEGs and then do WGCNA?

Regards

Answer 1

[Benn]

A good way to start is reading the manual of limma. Read about the RNA-seq methods and follow some of the case studies. Good luck.

[naseerkhan861]

Thanks Benn for your reply, I am reading limma, Thank you very much indeed!