miRWalk and Cytoscape to show microRNA-mRNA interactions

Question

[Shicheng Guo]

Hi All,

I have about 5 miRNAs and each of them I have some confirmed mRNA target, I want to use the following figure to show the network among these miRNA and mRNA. Is there any protocol?

Thanks.

[Leite]

Dear @Shicheng Guo

Do you already have mapped the targets?

If so, create a txt file with the miRNA-target and import into cytoscape.

After that you will need to read a little in cytoscape's manual and use your creativity.

You can also see a brief explanation of how to deal with cytoscape here:

A: Cytoscape network of upregulate and downregulate transcription factors: how to d

If you have not mapped the targets....

You can get some insights here:

C: MicroRNA-target interactions databases

[Shicheng Guo]

Thanks Leite, Your suggestion is really helpful!!