Seurat nFeature_RNA filter number in human

Question

[yueli7]

Hello, Anyone,

This command in Seurat package means that :

We filter cells that have over 2,500 or less than 200, >5% mitochondrial counts.

If I worked on human, how many unique feature counts we have to filter?

Thanks in advance for great help!

Best,

Yue

pbmc <- subset(pbmc, subset = nFeature_RNA > 200 & nFeature_RNA < 2500 & percent.mt < 5)

[ATpoint]

Why not sticking to what the tutorial suggests if you have no experience?

[yueli7]

Hello, ATpoint,

Thank you for your response！

There is no suggestion in the tutorial:

https://satijalab.org/seurat/v3.1/pbmc3k_tutorial.html

best,

Yue

[yueli7]

Seurat allows you to easily explore QC metrics and filter cells based on any user-defined criteria.