How to create Illumina sample sheet file for single index data?

Question

[lakhujanivijay]

Hi All

I need help regarding how to format the sample sheet file (required for demultiplexing using bcl2fastq) for single index data. Usually I have dual index data and I keep below column names in the sample sheet.

• Sample_ID

• Sample_Name

• Sample_Plate

• Sample_Well

• I7_Index_ID

• index

• I5_Index_ID

• index2

• Sample_Project

• Description

---

Now, this time I have single index data and I am wondering whether I need to -

[1] Keep the i5_index_ID & index2 columns but keep them blank

OR

[2] Keep the i5_index_ID & index2 columns and add some text (NA)

OR

[3] Delete i5_index_ID & index2 columns

Please help.

Answer 1

[Devon Ryan]

Remove the I5_Index_ID and index2 columns. BTW, you can always remove Sample_Plate and Sample_Well.

[lakhujanivijay]

That solved the issue. Thanks Devon Ryan

Answer 2

[GenoMax]

You can download Illumina experiment manager software (Windows only). It allows you to make correct samplesheets for all Illumina kits/sequencers.