When I use the function fpkmSCVPlot via cummeRbund, I get one of the lines is horizontal

Question

[wukunouc]

When I analysis the differentially expressed gene , I used cummeRbund. I want to see the CV2 via the function fpkmSCVPlot, But I get one of the lines is horizontal, how can I interpret ？

I used command：

library(cummeRbund)
cuff<-readCufflinks("F:\\difference"
genes.scv<-fpkmSCVPlot(genes(cuff))
genes.scv

[ATpoint]

Do you have replicates? Please show the plot.

[wukunouc]

thank you for your attention, I have two conditions ,and each condition has four replicates

[Kristoffer Vitting-Seerup]

Please see: How to add images to a Biostars post

[ATpoint]

That link is broken or incomplete. Please follow the instructions linked by kristoffer.vittingseerup. Yu need the full link including the suffix (e.g. .png) and paste this into the image button field.