tBLASTn - How do I turn all repetitive masking off??
0
0
Entering edit mode
5.0 years ago
molly77 ▴ 10

Hi,

With tBLASTn v2.7.1+ on the command line: I'm using a transcriptome as my nucleotide database and using translated queries that are highly repetitive (these queries sequences have come from my transcriptome so I know they are there), and I'm not getting any hits.

How do I go about generating hits using repetitive queries to identify repetitve fragments in my transcriptome??? I used the "soft_masking false" parameter but it doesn't help. How do I turn off all forms of masking?

I have Google'd this for what seems like hours and cannot find any answers. The BLAST manual isn't that informative and can be confusing.

Thanks!

RNA-Seq blast tblastn Assembly • 812 views
ADD COMMENT

Login before adding your answer.

Traffic: 2098 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6