Is it possible to multiplex mora than 384 samples on Novaseq 6000 for a very low pass (0.5-10x) WGS?

Question

[Biomed]

Is it possible to multiplex mora than 384 samples on Novaseq 6000 for a very low pass (0.5-10x) WGS?

Answer 1

[ATpoint]

Yes, if you have enough barcodes there’s no technical limitation. Also see this somewhat related question:

Splitting RNA-Seq data set on two (three) flow cells

Take-Home message: You can pool as many samples as long as you have enough unique primer/index combinations.

[dawn.obermoeller]

PerkinElmer has a set of 1536 NEXTFLEX UDI Adapters for ultra high-throughput multiplexing on Illumina flow cells.