get the barcode file

Question

[yueli7]

Hello,

I tried to get the barcode file of SRR7191904.

Thanks in advance for any help!

Best,

Yue

fastq-dump --gzip --split-files SRR7191904.sra

bin$ head -n 10 SRR7191904_1.fastq
@SRR7191904.1 1 length=27
GATCGNGGTCCTGCTTCGCCTATGTCT
+SRR7191904.1 1 length=27
/AAAA#EEEEEEEEEEEEEEEEEEEEA
@SRR7191904.2 2 length=27
CACACNTCAATCTGCAGCAAAATTCAT
+SRR7191904.2 2 length=27
AAA6A#/AAEEEAEEEEAEEAEEEEEE
@SRR7191904.3 3 length=27
CTAGCNTAGCTACCGCGCTTGGATTTT

Answer 1

[Devon Ryan]

The data has been demultiplexed, so there is no traditional Illumina barcode file. The 10x cell barcode and UMI fastq file is the read 1 file that you showed.

[yueli7]

Hello, Cevon Ryan,

Thank yo so much for your great help!

Best,

Yue