How to do pacbio iso-seq analysis begin with NCBI SRA fastq data

Question

[FeiZhao]

Hi,

I have downloaded some public data PRJNA427246 from SRA database. The paper is “Hybrid Sequencing Reveals Insight into Heat Sensing and Signaling of Bread Wheat.”. https://doi.org/10/ggffjg. The sra data information are as follow:

When the download is complete, I convert the sra files into fastq files use fastq-dump. Now I want to analyze these fastq data, however, many analysis pipeline and methods are start from bam file. I would like to ask how to start the analysis from the fastq file downloaded from SRA database.

Best wishes

Answer 1

[tothepoint]

Convert fastq file in to sam file format and than bam file format. samtool will help

[FeiZhao]

the pacbio bam file have many orther information, just cnvert fastq file in to sam file?