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4.9 years ago
fiona.newberry
▴
80
I am using minimus2 to merge overlapping contigs to improve my assembly.
I am getting the following error from minimus2 in .runAmos.log
CONSTRUCTIONTIME /opt/software/conda_env/opt/mummer-3.23/mummer PHE_1_CDHIT.ntref 29.47
/opt/software/conda_env/opt/mummer-3.23/mummer: cannot open file "1.qry.seq" or file "1.qry.seq" is empty
ERROR: mummer and/or mgaps returned non-zero
!!! 2019-12-19 15:36:28 Command: /opt/software/conda_env/bin/nucmer -maxmatch -c 40 1.ref.seq PHE_1_CDHIT.qry.seq -p PHE_1_CDHIT exited with status: 1
!!! END - Elapsed time: 0d 0h 0m 37s
These are the output files it is making:
1.mgaps
1.ref.seq
1.ntref
1.runAmos.log
1.qry.seq
And one directory:
1.bnk
Any help would be appreciated.