Entering edit mode
12.8 years ago
Pablo Marin-Garcia
★
2.0k
flicker reports normalized hits (HNA) but tools like DESeq (http://www-huber.embl.de/users/anders/DESeq/) uses for their analysis raw data (from tables with integers).
When I look to the mir/xxx.hit.txt it looks like:
mir.fa/rno-let-7a 8975.83333333333 630.2373006394
mir.fa/rno-let-7a-1* 3 0.210644720295408
mir.fa/rno-let-7b 1908.83333333333 134.028554530184
mir.fa/rno-let-7b* 4 0.280859627060544
mir.fa/rno-let-7c 60.3333333333333 4.23629937482988
mir.fa/rno-let-7c-2* 3 0.210644720295408
mir.fa/rno-let-7d 571 40.0927117628927
mir.fa/rno-let-7d* 506 35.5287428231588
mir.fa/rno-let-7e 135 9.47901241329337
mir.fa/rno-let-7e* 1 0.070214906765136
Is there any place in the outputs of flicker where the number of possible mappings of a read is stored so I can turn the HNA into raw count? Or is there a way of running flicker again not having the multiple mapping normalized?
Thanks Lars for the tag editing.