Add signatures (trinucleotide context) to vcf file
0
0
Entering edit mode
4.8 years ago
graeme.thorn ▴ 100

I have lots of (single) VCF files from a capture library which I notice are contaminated with what appear to be a lot of 8-oxoguanine artefacts (C>A and G>T) from the capture. Following from this paper https://www.ncbi.nlm.nih.gov/pubmed/23303777, I want to reduce the prevalence of these artefacts using a similar method, which means that I need to attach the trinucleotide context to each line of the vcf.

I can see plenty of packages which can summarise the context and signatures, but for filtering purposes it needs to be added to each entry so I can filter line-by-line. Is there a package/script which will allow me to manipulate the vcf directly to add these?

variants • 962 views
ADD COMMENT

Login before adding your answer.

Traffic: 2595 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6