Primer-Blast results "Exception error: No alias or index file found for nucleotide database"

Question

[saraG]

Dear all,

I am picking up primers through NCBI Primer-BLAST and it keeps returning the following error:

Exception error: No alias or index file found for nucleotide database [repeat/repeat_9989] in search path [/export/home/service/grid_nodes/primertool:/netmnt/blast/db/disk.blast/blast2150:/net/frosty/vol/blast/db/blast:] .

I am not sure it has anything to do with my parameters because I have tried the same genes and options previously and it has worked. I' ve looked through the forum and similar post only talk of this problem in BLAST+, but not on the web. It might be a temporary problem, but any idea of what might be going on? Thank you very much for your help.

[saraG]

Sorry, the screenshot link is this one https://ibb.co/yQBYQ7K

Answer 1

[Joe]

My guess would be that it may be a temporary problem, but this is likely something you'd need to speak to NCBI's helpdesk about, rather than the forum.

[saraG]

Hi Joe, You were right, Primer-Blast is working again! Many thanks for the advice anyway, I will contact NCBI help desk next time.