How to functional annotation bins (the output of MaxBin) of metagenomics

Question

[ttluong2]

Hi all, I am working with shotgun metagenomics. I got 2 bins from MaxBin (fasta file). Now, I want to annotation the bins. I tried to use RAST but keep having error. I don't know why and also don't know which platforms can help me annotation bins (output of MaxBin). I am a beginner of such analysis. Please help me. THank you in advance.

Answer 1

[onestop_data]

Hm... very odd. I wonder if RAST is having some problem with the bin contamination. Maybe run CheckM and show how complete/contaminated your bins are.

Also, an alternative for microbial genome annotation is to use prokka.