Plink Error in PCA plot

Question

[tothepoint]

Hello Everyone. I am working on gwas data and processed samples from 7 different varieties with 70+ sample size of pig. Currently I am trying to make PCA plot by Plink plink2 --bfile data --pca --allow-extra-chr

I am getting an error: "At least 2 people required for pairwise analysis." Please suggest what i am doing wrong and how to fix the issue. I will be grateful to you.

[chrchang523]

Please post the entire .log file.

[tothepoint]

I am getting this

102565458 variants loaded from .bim file.
1 person (0 males, 0 females, 1 ambiguous) loaded from .fam.
Ambiguous sex ID written to plink.nosex .
Error: At least 2 people required for pairwise analysis.

[chrchang523]

That isn’t the entire .log file, but it’s enough. Why do you expect this to work? The file contains only one sample, not 70.

[Hernán]

First report the exact PLINK version you're using with plink2 --version

Count the lines of your .ped file (you may generate the ped file from bim with plink).

wc -l data.ped

That should be enough to see what's happening.

You can check the file formats by doing

file data.*