Entering edit mode
4.8 years ago
taoi2
▴
40
After RNA-seq paired-end-reads are aligned with STAR, I want to calculate expression levels using RSEM.
The STAR index was built on .gtf and .fa downloaded from human GENCODE.
Can "rsem-calculate-expression" command be run with both "Aligned.toTranscriptome.out.bam" files from STAR and the above STAR index ?
Or
Must I create an index for RSEM and use the index in stead of the above STAR index ?