MetaBat 2 does not produce a bin while running ok

Question

[caroline.monteil]

Hi,

Trying to get contigs binned with MetaBat2, everything is doing ok, a bin folder is created, the .txt files are produced. But no bin.fa is added to folder.

Importantly, contigs were obtained from single cell amplified DNA. it possible that the assembly represents one genome only. Would it explain the situation?

Thanks

Caroline

Answer 1

[onestop_data]

I guess it means that Metabat2 somehow was not able to bin any of your contigs. What did you use to create your BAM files prior to running it? did you make sure it was a sorted BAM?

I ran the tool today on a simulated dataset with 10 species, and it was only able to bin 2 of them. I guess in your cause it did not bin anything.

Try using CONCOCT. I had better luck with it