Question

How to covert RKPM into absolute quantification?

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4.7 years ago

marongiu.luigi ▴ 730

Hello, is it possible to convert RPKM values into absolute values? That is to convert a qualitative (relative) measurement into a quantitative (absolute) measurement? If I have an RPKM of, let's say, 375 for a gene of interest (or micro-organism) and the sample was 1 mL of something, can I calculate the number of initial copies of the gene?

sequencing genome alignment • 996 views

ADD COMMENT • link updated 4.7 years ago by Kevin Blighe 88k • written 4.7 years ago by marongiu.luigi ▴ 730

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If you don't have a spike-in of known concentration is impossible. See answers to a similar question here

ADD REPLY • link 4.7 years ago by Fabio Marroni ★ 3.0k

score 3 · Accepted Answer · 2020-03-01

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4.7 years ago

Devon Ryan 104k

This is not possible with regular RNA-seq data.

ADD COMMENT • link 4.7 years ago by Devon Ryan 104k