Usearch fatal error 'CharToIntQUal('M). phred score 44 out of range 0..42,base 33

Question

[hafiz.talhamalik]

I m trying to filter my metgenome's fastq reads using Usearch but I am facing this error.

fatal error 'CharToIntQUal('M). phred score 44 out of range 0..42,base 33

Any suggestion to overcome this ?

[Carambakaracho]

your phred scores are not the expetced Phred 33 base, see wikipedia on fastq for an overview

[hafiz.talhamalik]

thanks it helped me to resolve the issue

[jsarkarind]

hi... how you resolve the issue? I am facing same problem. I am sure about that the fastq file is OK because all iontorrent generated file shows the same problem..

[hafiz.talhamalik]

enable the tag -fastqmin 0 -fastqmax 47 while filtering. It will resolve the issue