How to exclude reads with five or more variations from the reference
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4.7 years ago
Gene ▴ 20

I am working on SNP analysis. Sequencing was performed by Molecular Inversion Probe (MIP)-based re-sequencing. I have BAM files right now, and I am trying to eliminate reads with more than five variations from the reference per read, with the exception of multi-basepair insertions and deletions.

I am student, beginner, and have not found a way to perform it. All ideas are welcomed.

Is there a tool to find a number of variations from the reference per read?

sequencing • 426 views
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