Uncommon wget behavior with ncbi genomes

Question

[flogin]

Hey guys, I'm trying to download all genomes of Eimeria, present on NCBI. So, as usually, I wrote this line:

wget -r --accept-regex ".*_genomic.fna.gz" "ftp://ftp.ncbi.nlm.nih.gov/genomes/refseq/protozoa/Eimeria*" -P .

But, none file was returned (I tried with -A insetead of --accept-regex too), but the same occurs.

I'm using GNU Wget 1.17.1, and other times I already download several genomes with the same line.

this is an example of a link ftp directory with the file that I want.

https://ftp.ncbi.nlm.nih.gov/genomes/refseq/protozoa/Eimeria_necatrix/latest_assembly_versions/GCF_000499385.1_ENH001/

the file in the case is: GCF_000499385.1_ENH001_genomic.fna.gz

Can anyone help ?

Answer 1

[vkkodali_ncbi]

You can use Entrez Direct for this as shown below:

esearch -db assembly -query 'Eimeria necatrix[organism]' \
| esummary \
| xtract -pattern DocumentSummary -element FtpPath_RefSeq \
| while read -r url ; do 
    path=$(echo $url | perl -pe 's/(GC[FA]_\d+.*)/\1\/\1_genomic.fna.gz/g') ; 
    wget -q --show-progress "$path" -P genome_data ; 
done

Alternatively, you can go to the NCBI Assembly portal, search for Eimeria necatrix[organism] and use the blue 'Download Assemblies' button to download the files of your choice.