how to use tblastn

Question

[sarahi_sanchez]

How can I run a local blastn? I am looking in contings for a protein. I just don't know what steps to take. tblastn.exe -query seq.fas -db database

[Ram]

What have you tried so far? Did you read the manual? Did you build the database?

[Asaf]

You're better off working in a linux environment. If you have access to a high-perfomance computer that will be great, if not you can use a virtual machine.

[Mehmet]

First you need to make a database using makeblastdb command Second, you can search your query against the database using tblastn