Question

Should I normalize TPM data?

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4.7 years ago

jt ▴ 20

Hi!

I want to use median gene level TPM by tissue from GTEx. Should I normalize it before using?

TPM gene • 1.6k views

ADD COMMENT • link 4.7 years ago by jt ▴ 20

score 1 · Answer 1 · 2020-04-03

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4.7 years ago

ATpoint 85k

TPM is already normalized for gene length and sequencing depth. If you want more explanation then please add details.

ADD COMMENT • link 4.7 years ago by ATpoint 85k

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Moved to answer

ADD REPLY • link 4.7 years ago by Kevin Blighe 88k