Pathway targeted genes

Question

[rob.costa1234]

I am working on to find NFkB targeted genes in a list of differentially expressed gene lists. My question is The genes from the DE gene list that are part of the NFkB pathway in a typical pathway anlaysis will be considered as the targets of NFKB pathway Or I have to look at some other databases. Any suggestion?

Answer 1

[gayachit]

If by targets you mean for further qPCR or such analysis then yes. You can confirm by doing classification using DAVID (https://david.ncifcrf.gov/). Take the list of significant DE genes and classify and check the NFKB pathway.