DNA-Seq and Whole Genome Sequencing, are they the same?
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4.6 years ago
rtyb91 ▴ 30

Dear members,

I have currently dispatched an order for whole genome sequencing in dogs using DNA extracted from whole blood using Qiagen DNEasy kit, and I was reading through publications to prepare for my analysis when the results return.

One question I have always gotten from my senior was what is the difference between DNA-sequencing and Whole Genome Sequencing, but I could never find any articles or publications differentiating them. It would be great if someone made like a tree on the difference between all the sequencing like RNA-seq, miRNA-seq and etc, but as of now it would be really appreciated if someone wise out there could enlighten me the differences between DNA-seq and WGS, and if you have a reference publication it would be even more awesome to share the link as well.

Thank you very much!

genome dna-seq whole genome sequencing • 1.1k views
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4.6 years ago
ATpoint 85k

DNA-seq is a summary term for all NGS-based techniques where the input material to construct the library is DNA. That can be WGS, but also ChIP-seq, ATAC-seq, WGBS/RRBS, MeDIPs, HiC, targeted exomes, etc.

WGS means that you perform DNA-seq with a library comprising the entire genome.

So every WGS is DNA-seq but not every DNA-seq is WGS.

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Thank you for the clarification! That clears the air immensely! Sorry for being such an amateur though, some how google searching and reading heaps of articles couldn't answer this question.

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Not add confusion but many of the methods noted by @ATPoint are still looking at genome-wide data but you are interested in specific regions.

For some analysis (e.g. transcriptome, targeted amplicons, exome sequencing) you are only looking at specific regions of the genome (as long as the experiment works right).

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No worries, if you are new to a topic then the common vocabularies sometimes sound like hieroglyphs, that is normal. I had the same with the statistical terms when I got started, but once you find a good explanation somewhere things start getting more clear.

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