Entering edit mode
12.6 years ago
Lee Katz
★
3.2k
Hi, I uploaded my own gene predictions via a genbank file to RAST (preview of the genbank file below). RAST changed every single gene except for some RNA genes to the forward direction.
When I used RAST without my gene predictions and uploaded only the assembly in fasta format, genes were correctly oriented.
Question: how would I alter my genbank file so that RAST respects the directionality of each gene?
LOCUS scaffold3/1 168998 bp dna linear BCT
DEFINITION XXXXX, unfinished sequence, whole genome shotgun sequence
ACCESSION unknown
KEYWORDS WGS.
SOURCE Vibrio cholerae
ORGANISM Vibrio cholerae
Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
Vibrionaceae; Vibrio.
FEATURES Location/Qualifiers
gene complement(942..259)
/locus_tag="2011EL-1144H_draft_0312"
CDS complement(942..259)
/locus_tag="2011EL-1144H_draft_0312"
/evidence=predicted
/translation="[removed for brevity here in biostar]"
/transl_table=11
/note="Predictors: gmhmmp, Glimmer3, BLAST"