Rast Genes All In The Forward Direction
0
0
Entering edit mode
12.6 years ago
Lee Katz ★ 3.2k

Hi, I uploaded my own gene predictions via a genbank file to RAST (preview of the genbank file below). RAST changed every single gene except for some RNA genes to the forward direction.

When I used RAST without my gene predictions and uploaded only the assembly in fasta format, genes were correctly oriented.

Question: how would I alter my genbank file so that RAST respects the directionality of each gene?

LOCUS       scaffold3/1           168998 bp    dna     linear   BCT
DEFINITION   XXXXX, unfinished sequence, whole genome shotgun sequence
ACCESSION   unknown
KEYWORDS    WGS.
SOURCE      Vibrio cholerae
  ORGANISM  Vibrio cholerae
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
FEATURES             Location/Qualifiers
     gene            complement(942..259)
                     /locus_tag="2011EL-1144H_draft_0312"
     CDS             complement(942..259)
                     /locus_tag="2011EL-1144H_draft_0312"
                     /evidence=predicted
                     /translation="[removed for brevity here in biostar]"
                     /transl_table=11
                     /note="Predictors: gmhmmp, Glimmer3, BLAST"
annotation • 1.9k views
ADD COMMENT

Login before adding your answer.

Traffic: 1866 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6