What is the minimum sample size to get reliable results in haplotype and nucleotide estimations?

Question

[apl00028]

Different software have different methods for estimating diversity and each method will have assumptions about the quantity of data required to produce a reliable result. I am estimating the haplotype and nucleotide diversity by DnaSP6 program of Sanger sequences. I would like what is the minimum sample size requires to get reliable results.

Thanks in advance.