Dear All,
I have Illumina array probe metadata with me HumanHT-12_V3_0_R3_11283641_A.txt
. Now, I need to annotate the gene symbols present within them. Precisely, I need to know the biotype of all genes available in the Illumina metadata file.
The file consists of 48k genes. When I use GENCODE GTF
file (hg38). I can annotate 24k genes, and the rest is not annotated. In that case, I would like to know, whether there is any annotation file specific for Illumina?
The HEADER of the above-mentioned file consists of,
? Illumina, Inc.
[Heading]
Date 7/1/2010
ContentVersion 3.0
FormatVersion 1.0.0
Number of Probes 48803
Number of Controls 784
[Probes]
Species Source Search_Key Transcript ILMN_Gene Source_Reference_ID RefSeq_ID Unigene_ID Entrez_Gene_ID GI Accession Symbol Protein_Product Probe_Id Array_Address_Id Probe_Type Probe_Start Probe_Sequence Chromosome Probe_Chr_Orientation Probe_Coordinates Cytoband Definition Ontology_Component Ontology_Process Ontology_Function Synonyms Obsolete_Probe_Id
Currently, I extracted the ILMN_Gene
and tried to annotate it with the GENCODE GTF
file. However, I am not able to get the biotype of all genes within the Illumina file (48k).
Any suggestions would be of great help Thanks!