Mismatch table from pariwise sequence alignment
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4.6 years ago
MAPK ★ 2.1k

This must be a simple problem and possibly there are tools available to do this. I need to create a table with SNP and reference position where those SNPs are located. I have a reference genome and contigs (in fasta) I want to check.

For example:

reference_genome: ATTGGTTGGGTTGGAAGGGGCCCA

contig: GTCGGGTTGGAGGGGGCCCA

So what I want is the position for T and A (mismatches in bold) with both reference and alternate nucleotides.

How can I get this done? Thanks for your help in advance.

alignment • 503 views
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