I'm just starting to learn how to interpret NGS results and this picture confused me, I can't really tell the difference between transcriptome sequencing and full exome sequencing. Can somebody explain how to distinguish them in this particular scheme and in general?
Exome sequencing on the DNA level means that you capture exonic regions prior to sequencing. The term exome sequencing on RNA level is uncommon. It is typically called RNA-seq and in it you sequence typically mRNA so mostly the spliced form of RNA which then only contains exons.
I do not know. Can be both. If I had to bet money I would probably go for RNA-seq since the first exons of the isoforms are poorly covered, therefore exome (DNA) is probably unlikely but this is wildly guessing. You have to know since you have the data.
Its RNA Sequencing. In exome sequencing, the library is enriched for exonic regions of the genome but it doesn't capture the exact splicing information of mRNAs (split reads mapped to exon-exon junctions). Exome sequencing reads need not necessarily span the exon-exon junctions because you are not sequencing mRNA, instead DNA enriched for exons.
Exome sequencing on the DNA level means that you capture exonic regions prior to sequencing. The term exome sequencing on RNA level is uncommon. It is typically called RNA-seq and in it you sequence typically mRNA so mostly the spliced form of RNA which then only contains exons.
So in terms of this particular illustration is it a transcriptome or exome?
I do not know. Can be both. If I had to bet money I would probably go for RNA-seq since the first exons of the isoforms are poorly covered, therefore exome (DNA) is probably unlikely but this is wildly guessing. You have to know since you have the data.
Edit: Ok, what geek_y says :)