Marker genes of a scRNA-Seq experiment not found in the processed data set gene symbols
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4.6 years ago

Hello,

I have downloaded the processed data of gene expression of a scRNA-Seq experiment (Tirosh et al, 2016). In their supplementary data they have listed marker genes for each cell type. But when I search these markers in the processed expression data (text file), they are not found. Do you know what seems to be the problem? I want to use these marker genes for tSNE of cell types.

Thanks,

RNA-Seq • 1.1k views
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Can you mention the GEO number for that dataset. What method was used for single cell?

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the GEO accession number is GSE72056 and they used Illumina NextSeq 500 for sequencing.

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I meant what single cell sequencing technology.

The file has 23650+ genes. Don't know what specific genes you could find. Try the grep command.

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