ERROR: "ascp: Failed to open TCP connection for SSH, exiting."

Question

[dpc]

Dear bioinformaticians, I was trying to download FASTQ file (ERR036000) from ENA via aspera connect. I used the following command:

**ascp -QT -l 300m -P33001 -i home/deep/.aspera/connect/etc/asperaweb_id_dsa.openssh [e-mail]:/vol1/fastq/ERR036/ERR036000/ERR036000_1.fastq.gz .**

BUT, got the following response:

**ascp: Failed to open TCP connection for SSH, exiting.**

Please tell me where is the problem?

Thank You

[GenoMax]

Failed to open TCP connection for SSH

Since @ATPoint is able to get Aspera to work this ss very likely indicative of local firewall controls preventing Aspera from connecting. Please look at that first.

Answer 1

[ATpoint]

Hello DEEP!

I told you in the previous thread that ENA is currently down.

Problem while downloading SRR file via **ASPERA CONNECT**

EDIT: 06.05.2020: For me ENA is up and running.

[rob234king]

is it up and running as I'm having the same problem

[kzkedzierska]

I am having the same problem, so I bet it's down? but nothing on Twitter and their website.

Did you manage to make it work?

[dpc]

I have tried today once again. And getting the same ERROR.

[ATpoint]

Can you share a download link? A just downloaded a random file from ENA at 223Mb/s without errors.

[dpc]

Hi sir... I've read your tutorial in biostars regarding download by aspera connect through sra-explorer. I am downloading using the ascp link generated. But I am getting only 1.7mbps around speed. Although it shows 14mbps speed, I've seen only 1GB is downloaded in 10 minutes. Do you have any suggestion?

[ATpoint]

You probably have a slow internet connection. Not much you can do about this.

[dpc]

Okay sir... One thing is I've not configured the proxy settings (http and gasp) in aspera after installation. May this be the cause?