How to test transposition in BAM files?

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4.6 years ago

marongiu.luigi ▴ 730

Hello,

is there a good pipeline for the analysis of transposition using WGS data? In other words, that is the procedure to assess whether a transposon has jumped between one sample and another?

Thank you

Assembly sequencing trasnposition • 1.0k views

ADD COMMENT • link 4.6 years ago by marongiu.luigi ▴ 730

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I think you need to explain a bit more in detail what exactly it is you're looking for and what type of data you have.

ADD REPLY • link 4.6 years ago by Friederike 9.0k

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A quick search in PubMed led to a couple of review papers on the subject -- have you perused those?

e.g. https://mobilednajournal.biomedcentral.com/articles/10.1186/s13100-015-0055-3, https://pubmed.ncbi.nlm.nih.gov/26025622/, https://pubmed.ncbi.nlm.nih.gov/26025622/, https://pubmed.ncbi.nlm.nih.gov/29390960/

ADD REPLY • link 4.6 years ago by Friederike 9.0k

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These seem promising, I'll read them, thank you.

ADD REPLY • link 4.6 years ago by marongiu.luigi ▴ 730

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This seems like a very fitting (and very recent) publication, too:

"Mobile Genomics: Tools and Techniques for Tackling Transposons"

ADD REPLY • link 4.6 years ago by Friederike 9.0k

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