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4.5 years ago
devbt15
▴
30
Dear all, Recently I performed RNASeq 150 bp paired-ends reads in rice (Illumina). I performed fastqc on the fastq files and obtained %GC plots (in the link below):
As shown in the plot, a second bump is observed at 70%. However, fastqc does not suggest any overrepresented sequences or adaptor contamination.
Any suggestions on what these could be?
Regards.
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Have you checked to make sure that there is no rRNA contamination in your data. Has the data been trimmed?