FASTQC result FAILED in adapter content and k-mer section!!!

Question

[dpc]

Hi there!!! I am analysing already submitted data in SRA. My fastqc result shows "No overrepresented sequences" but FAILS adapter content test and k-mer content test. why? Is it normal? If not, what should I do? Check the file here

Thanks, DC7

[GenoMax]

Please don't post similar content in multiple threads.

Start by reading FastQC blog posts from authors of FastQC that you will find at this link. FastQC is a QC program so a failure does not immediately mean that the data is bad. You need to look at the results of FastQC in context of the kind of data you are analyzing. e.g. ChIPseq, RNAseq data may show failures on over-represented sequences. But that is expected with those kinds of experiments.

[GenoMax]

Hello DC7!

Questions similar to yours can already be found at:

• FastQ check result

We have closed your question to allow us to keep similar content in the same thread.

If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.

Cheers!

[dpc]

That post is also mine. And I agreed to remove that. Thus, please don't remove this one. And also, do you have any idea about the problem?

[GenoMax]

I will close the other post.