ORFs finding tools

0

Entering edit mode

4.5 years ago

profnf11295 • 0

Hello,,

I'm working on a project with ORFs of 10 bacterial genomes, i extracted my ORFs using emboss getorf tool then i looked for an overlap between ORFs and CDS of those bacterial genomes, i've found almost 65% of ORFs overlap with CDS at either start codon or end codon and scientifically in prokaryotes it's abnormal .. so is there anyone have any possible explanation ? and what is exactly the difference between emboss getorf and ncbi getorf ?

ORF CDS genome getorf findorf • 1.3k views

ADD COMMENT • link updated 4.0 years ago by Biostar 20 • written 4.5 years ago by profnf11295 • 0

0

Entering edit mode

Can you clarify if these are new genomes or genomes that have been newly sequenced? If latter, you may want to use a program like prokka (LINK) instead of simple ORF programs.

ADD REPLY • link 4.5 years ago by GenoMax 147k

0

Entering edit mode

Also, please try to support us with the command parameters that you used. Make sure that you selected Bacteria when you run the program.

ADD REPLY • link 4.5 years ago by Medhat 9.8k

Login before adding your answer.