Entering edit mode
4.5 years ago
tianshenbio
▴
180
I am now using command-line RNAhybrid package to predict miRNA targets using the following command:
RNAhybrid -c -p 0.05 -m 10000 -s 3utr_fly -t my_utr.fa -q my_miR.fa
There is a species option ‘-s’ to tell RNAhybrid to quickly estimate statistical parameters, assuming the 3UTR are from either human, worm, or fly. ?I used fly since I am working with a non-model butterfly species, and is the cloasest species...Is this appropriate? Is there a way I can avoid choosing from the three species?
